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dbiblast |
Wiki
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Function
Index a BLAST databaseDescription
dbiblast indexes a blast database created by the NCBI indexing programs formatdb, pressdb or setdb, and builds EMBL CD-ROM format index files. This format is used by the software on the EMBL database CD-ROM distribution and by the Staden package in addition to EMBOSS, and appears to be the most generally used and publicly available index file format.The index files of the blast database format are not useful by themselves as they do not hold the entrynames or accession numbers, but they are used as pointers to the reference and sequence information in the database.
Because there are two sequence types (nucleic acid and protein) and two index formats (blast1 from pressdb or setdb, and blast2 from formatdb) with different index file names, dbiblast will ask about the database type. If the answer is "unknown" dbiblast will test each possible set of file names until one is found.
Usage
Here is a sample session with dbiblastThis creates EMBOSS indices for the BLAST database 'swnew'
% dbiblast
Index a BLAST database
Database name: swnew
Database directory [.]: data
Wildcard database filename [swnew]:
Release number [0.0]:
Index date [00/00/00]:
N : nucleic
P : protein
? : unknown
Sequence type [unknown]:
1 : wublast and setdb/pressdb
2 : formatdb
0 : unknown
Blast index version [unknown]:
General log output file [outfile.dbiblast]:
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Go to the output files for this example
Command line arguments
Index a BLAST database
Version: EMBOSS:6.6.0.0
Standard (Mandatory) qualifiers:
[-dbname] string Database name (Any string from 2 to 19
characters, matching regular expression
/[A-z][A-z0-9_]+/)
-directory directory [.] Database directory
-filenames string [Database name] Wildcard database filename
(Any string)
-release string [0.0] Release number (Any string up to 9
characters)
-date string [00/00/00] Index date (Date string dd/mm/yy)
-seqtype menu [unknown] Sequence type (Values: N
(nucleic); P (protein); ? (unknown))
-blastversion menu [unknown] Blast index version (Values: 1
(wublast and setdb/pressdb); 2 (formatdb); 0
(unknown))
-outfile outfile [*.dbiblast] General log output file
Additional (Optional) qualifiers: (none)
Advanced (Unprompted) qualifiers:
-fields menu [acc] Index fields (Values: acc (acnum
accession number index); sv (seqvn sequence
version and gi number index); des (des
description index))
-exclude string Wildcard filename(s) to exclude (Any string)
-sortoptions string [-T . -k 1,1] Sort options, typically '-T .'
to use current directory for work files and
'-k 1,1' to force GNU sort to use the first
field (Any string)
-maxindex integer [0] Maximum index length (Integer 0 or more)
-[no]systemsort boolean [Y] Use system sort utility
-[no]cleanup boolean [Y] Clean up temporary files
-sourcefile boolean Use FASTA source file
-indexoutdir outdir [.] Index file output directory
Associated qualifiers:
"-directory" associated qualifiers
-extension string Default file extension
"-outfile" associated qualifiers
-odirectory string Output directory
"-indexoutdir" associated qualifiers
-extension string Default file extension
General qualifiers:
-auto boolean Turn off prompts
-stdout boolean Write first file to standard output
-filter boolean Read first file from standard input, write
first file to standard output
-options boolean Prompt for standard and additional values
-debug boolean Write debug output to program.dbg
-verbose boolean Report some/full command line options
-help boolean Report command line options and exit. More
information on associated and general
qualifiers can be found with -help -verbose
-warning boolean Report warnings
-error boolean Report errors
-fatal boolean Report fatal errors
-die boolean Report dying program messages
-version boolean Report version number and exit
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| Qualifier | Type | Description | Allowed values | Default | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Standard (Mandatory) qualifiers | ||||||||||
| [-dbname] (Parameter 1) |
string | Database name | Any string from 2 to 19 characters, matching regular expression /[A-z][A-z0-9_]+/ | Required | ||||||
| -directory | directory | Database directory | Directory | . | ||||||
| -filenames | string | Wildcard database filename | Any string | Database name | ||||||
| -release | string | Release number | Any string up to 9 characters | 0.0 | ||||||
| -date | string | Index date | Date string dd/mm/yy | 00/00/00 | ||||||
| -seqtype | list | Sequence type |
|
unknown | ||||||
| -blastversion | list | Blast index version |
|
unknown | ||||||
| -outfile | outfile | General log output file | Output file | <*>.dbiblast | ||||||
| Additional (Optional) qualifiers | ||||||||||
| (none) | ||||||||||
| Advanced (Unprompted) qualifiers | ||||||||||
| -fields | list | Index fields |
|
acc | ||||||
| -exclude | string | Wildcard filename(s) to exclude | Any string | |||||||
| -sortoptions | string | Sort options, typically '-T .' to use current directory for work files and '-k 1,1' to force GNU sort to use the first field | Any string | -T . -k 1,1 | ||||||
| -maxindex | integer | Maximum index length | Integer 0 or more | 0 | ||||||
| -[no]systemsort | boolean | Use system sort utility | Boolean value Yes/No | Yes | ||||||
| -[no]cleanup | boolean | Clean up temporary files | Boolean value Yes/No | Yes | ||||||
| -sourcefile | boolean | Use FASTA source file | Boolean value Yes/No | No | ||||||
| -indexoutdir | outdir | Index file output directory | Output directory | . | ||||||
| Associated qualifiers | ||||||||||
| "-directory" associated directory qualifiers | ||||||||||
| -extension | string | Default file extension | Any string | |||||||
| "-outfile" associated outfile qualifiers | ||||||||||
| -odirectory | string | Output directory | Any string | |||||||
| "-indexoutdir" associated outdir qualifiers | ||||||||||
| -extension | string | Default file extension | Any string | |||||||
| General qualifiers | ||||||||||
| -auto | boolean | Turn off prompts | Boolean value Yes/No | N | ||||||
| -stdout | boolean | Write first file to standard output | Boolean value Yes/No | N | ||||||
| -filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N | ||||||
| -options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N | ||||||
| -debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N | ||||||
| -verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y | ||||||
| -help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N | ||||||
| -warning | boolean | Report warnings | Boolean value Yes/No | Y | ||||||
| -error | boolean | Report errors | Boolean value Yes/No | Y | ||||||
| -fatal | boolean | Report fatal errors | Boolean value Yes/No | Y | ||||||
| -die | boolean | Report dying program messages | Boolean value Yes/No | Y | ||||||
| -version | boolean | Report version number and exit | Boolean value Yes/No | N | ||||||
Input file format
Any BLAST1 or BLAST2 database index files.
Output file format
Output files for usage example
File: division.lkp
This file contains non-printing characters and so cannot be displayed here.
File: entrynam.idx
This file contains non-printing characters and so cannot be displayed here.
File: acnum.hit
This file contains non-printing characters and so cannot be displayed here.
File: acnum.trg
This file contains non-printing characters and so cannot be displayed here.
File: outfile.dbiblast
########################################
# Program: dbiblast
# Rundate: Mon 15 Jul 2013 12:00:00
# Dbname: swnew
# Release: 0.0
# Date: 15/07/13
# CurrentDirectory: /homes/user/test/qa/dbiblast-ex-keep/
# IndexDirectory: ./
# IndexDirectoryPath: /homes/user/test/qa/dbiblast-ex-keep/
# Maxindex: 0
# Fields: 2
# Field 1: id
# Field 2: acc
# Directory: /homes/user/test/data/
# DirectoryPath: /homes/user/test/data/
# Filenames: swnew
# Exclude:
# Files: 1
# File 1: /homes/user/test/data/swnew
########################################
# Commandline: dbiblast
# -dbname swnew
# -directory ../../data
########################################
filename: '/homes/user/test/data/swnew'
id: 9
acc: 9
Index acc: maxlen 6 items 9
Total 1 files 9 entries (0 duplicates)
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dbiblast creates four index files. All are binary but with a simple format.
- division.lkp is the master index file, and has a 300 byte header
containing the database name and date plus information on the record
size. This header is followed by one record for each database file,
giving the full file name for the data file, and optionally a second
sequence file.
- entryname.idx is the entry name index. It has the same 300 byte header, mainly used to store the record size which will depend on the size of the longest entryname in the database. Each entry is stored in sorted alphanumeric order so that a binary search can be used to efficiently find any record. The record also holds the file number from division.lkp and the offsets in the data and sequence files for that entry.
- acnum.trg holds the accession number information. The file has the usual 300 byte header, and a sorted list of record by accession number. Each accession number record contains the first record number in acnum.hit and the total number of records in acnum.hit so that secondary (duplicated) accession numbers can be searched.
- acnum.hit is a very simple file. After the usual 300 byte header, each record simply holds the record number in entryname.idx. An accession number search will use acnum.trg to find a start position and number of records to read in this file, and will then simply read the entryname.idx records for each entry in turn.
Data files
None.Notes
dbiblast can't use the new style of Blast indices. You must create the old style of Blast indices by adding -A F to the formatdb command line.Having created the EMBOSS indices for this file, a database can then be defined in the file emboss.defaults as something like:
DB swnew [ type: P format: embl method: blast directory: /data/embl ]
Fields Indexed
By default, dbiblast will index the ID name and the accession number (if present).If they are present in your database, you may specify that dbiblast should index the Sequence Version and GI number and the words in the description by using the '-fields' qualifier with the appropriate values.
Indexing the Blast database
dbiblast can't use the new style of Blast indices. You must create the old style of Blast indices by adding -A F to the formatdb command line.The reason is that NCBI have not documented the new format, so we cannot extend dbiblast to read it.
If you have the original (fasta format) file you can build the previous blast database format with:
formatdb -A F
This turns off the new "ASN.1" formatting. blastall will still work.
References
None.Warnings
Diagnostic Error Messages
None.Exit status
None.Known bugs
dbiblast can't use the new style of Blast indices. You must create the old style of Blast indices by adding -A F to the formatdb command line.See also
| Program name | Description |
|---|---|
| dbifasta | Index a fasta file database |
| dbiflat | Index a flat file database |
| dbigcg | Index a GCG formatted database |
| dbxcompress | Compress an uncompressed dbx index |
| dbxedam | Index the EDAM ontology using b+tree indices |
| dbxfasta | Index a fasta file database using b+tree indices |
| dbxflat | Index a flat file database using b+tree indices |
| dbxgcg | Index a GCG formatted database using b+tree indices |
| dbxobo | Index an obo ontology using b+tree indices |
| dbxreport | Validate index and report internals for dbx databases |
| dbxresource | Index a data resource catalogue using b+tree indices |
| dbxstat | Dump statistics for dbx databases |
| dbxtax | Index NCBI taxonomy using b+tree indices |
| dbxuncompress | Uncompress a compressed dbx index |
Author(s)
Peter RiceEuropean Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.